A Diverse Virome of Leafroll-Infected Grapevine Unveiled by dsRNA Sequencing.
Identifieur interne : 000110 ( Main/Exploration ); précédent : 000109; suivant : 000111A Diverse Virome of Leafroll-Infected Grapevine Unveiled by dsRNA Sequencing.
Auteurs : Mamadou L. Fall [Canada] ; Dong Xu [Canada] ; Pierre Lemoyne [Canada] ; Issam E Ben Moussa [Canada] ; Carole Beaulieu [Canada] ; Odile Carisse [Canada]Source :
- Viruses [ 1999-4915 ] ; 2020.
Abstract
Quebec is the third-largest wine grape producing province in Canada, and the industry is constantly expanding. Traditionally, 90% of the grapevine cultivars grown in Quebec were winter hardy and largely dominated by interspecific hybrid Vitis sp. cultivars. Over the years, the winter protection techniques adopted by growers and climate changes have offered an opportunity to establish V. vinifera L. cultivars (e.g., Pinot noir). We characterized the virome of leafroll-infected interspecific hybrid cultivar and compared it to the virome of V. vinifera cultivar to support and facilitate the transition of the industry. A dsRNA sequencing method was used to sequence symptomatic and asymptomatic grapevine leaves of different cultivars. The results suggested a complex virome in terms of composition, abundance, richness, and phylogenetic diversity. Three viruses, grapevine Rupestris stem pitting-associated virus, grapevine leafroll-associated virus (GLRaV) 3 and 2 and hop stunt viroid (HSVd) largely dominated the virome. However, their presence and abundance varied among grapevine cultivars. The symptomless grapevine cultivar Vidal was frequently infected by multiple virus and viroid species and different strains of the same virus, including GLRaV-3 and 2. Our data show that viruses and viroids associated with the highest number of grapevines expressing symptoms included HSVd, GLRaV-3 and GLRaV-2, in gradient order. However, co-occurrence analysis revealed that the presence of GLRaV species was randomly associated with the development of virus-like symptoms. These findings and their implications for grapevine leafroll disease management are discussed.
DOI: 10.3390/v12101142
PubMed: 33050079
PubMed Central: PMC7599845
Affiliations:
Links toward previous steps (curation, corpus...)
Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">A Diverse Virome of Leafroll-Infected Grapevine Unveiled by dsRNA Sequencing.</title><author><name sortKey="Fall, Mamadou L" sort="Fall, Mamadou L" uniqKey="Fall M" first="Mamadou L" last="Fall">Mamadou L. Fall</name><affiliation wicri:level="1"><nlm:affiliation>Saint-Jean-sur-Richelieu Research and Development Centre, Agriculture and Agri-Food Canada, St-Jean-sur-Richelieu, Quebec, QC J3B 3E6, Canada.</nlm:affiliation><country xml:lang="fr">Canada</country><wicri:regionArea>Saint-Jean-sur-Richelieu Research and Development Centre, Agriculture and Agri-Food Canada, St-Jean-sur-Richelieu, Quebec, QC J3B 3E6</wicri:regionArea><wicri:noRegion>QC J3B 3E6</wicri:noRegion></affiliation></author><author><name sortKey="Xu, Dong" sort="Xu, Dong" uniqKey="Xu D" first="Dong" last="Xu">Dong Xu</name><affiliation wicri:level="1"><nlm:affiliation>Saint-Jean-sur-Richelieu Research and Development Centre, Agriculture and Agri-Food Canada, St-Jean-sur-Richelieu, Quebec, QC J3B 3E6, Canada.</nlm:affiliation><country xml:lang="fr">Canada</country><wicri:regionArea>Saint-Jean-sur-Richelieu Research and Development Centre, Agriculture and Agri-Food Canada, St-Jean-sur-Richelieu, Quebec, QC J3B 3E6</wicri:regionArea><wicri:noRegion>QC J3B 3E6</wicri:noRegion></affiliation></author><author><name sortKey="Lemoyne, Pierre" sort="Lemoyne, Pierre" uniqKey="Lemoyne P" first="Pierre" last="Lemoyne">Pierre Lemoyne</name><affiliation wicri:level="1"><nlm:affiliation>Saint-Jean-sur-Richelieu Research and Development Centre, Agriculture and Agri-Food Canada, St-Jean-sur-Richelieu, Quebec, QC J3B 3E6, Canada.</nlm:affiliation><country xml:lang="fr">Canada</country><wicri:regionArea>Saint-Jean-sur-Richelieu Research and Development Centre, Agriculture and Agri-Food Canada, St-Jean-sur-Richelieu, Quebec, QC J3B 3E6</wicri:regionArea><wicri:noRegion>QC J3B 3E6</wicri:noRegion></affiliation></author><author><name sortKey="Moussa, Issam E Ben" sort="Moussa, Issam E Ben" uniqKey="Moussa I" first="Issam E Ben" last="Moussa">Issam E Ben Moussa</name><affiliation wicri:level="1"><nlm:affiliation>Saint-Jean-sur-Richelieu Research and Development Centre, Agriculture and Agri-Food Canada, St-Jean-sur-Richelieu, Quebec, QC J3B 3E6, Canada.</nlm:affiliation><country xml:lang="fr">Canada</country><wicri:regionArea>Saint-Jean-sur-Richelieu Research and Development Centre, Agriculture and Agri-Food Canada, St-Jean-sur-Richelieu, Quebec, QC J3B 3E6</wicri:regionArea><wicri:noRegion>QC J3B 3E6</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:affiliation>Département de Biologie, Université de Sherbrooke, Sherbrooke, QC J1K 2R1, Canada.</nlm:affiliation><country xml:lang="fr">Canada</country><wicri:regionArea>Département de Biologie, Université de Sherbrooke, Sherbrooke, QC J1K 2R1</wicri:regionArea><wicri:noRegion>QC J1K 2R1</wicri:noRegion></affiliation></author><author><name sortKey="Beaulieu, Carole" sort="Beaulieu, Carole" uniqKey="Beaulieu C" first="Carole" last="Beaulieu">Carole Beaulieu</name><affiliation wicri:level="1"><nlm:affiliation>Département de Biologie, Université de Sherbrooke, Sherbrooke, QC J1K 2R1, Canada.</nlm:affiliation><country xml:lang="fr">Canada</country><wicri:regionArea>Département de Biologie, Université de Sherbrooke, Sherbrooke, QC J1K 2R1</wicri:regionArea><wicri:noRegion>QC J1K 2R1</wicri:noRegion></affiliation></author><author><name sortKey="Carisse, Odile" sort="Carisse, Odile" uniqKey="Carisse O" first="Odile" last="Carisse">Odile Carisse</name><affiliation wicri:level="1"><nlm:affiliation>Saint-Jean-sur-Richelieu Research and Development Centre, Agriculture and Agri-Food Canada, St-Jean-sur-Richelieu, Quebec, QC J3B 3E6, Canada.</nlm:affiliation><country xml:lang="fr">Canada</country><wicri:regionArea>Saint-Jean-sur-Richelieu Research and Development Centre, Agriculture and Agri-Food Canada, St-Jean-sur-Richelieu, Quebec, QC J3B 3E6</wicri:regionArea><wicri:noRegion>QC J3B 3E6</wicri:noRegion></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2020">2020</date><idno type="RBID">pubmed:33050079</idno><idno type="pmid">33050079</idno><idno type="doi">10.3390/v12101142</idno><idno type="pmc">PMC7599845</idno><idno type="wicri:Area/Main/Corpus">000020</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">000020</idno><idno type="wicri:Area/Main/Curation">000020</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Curation">000020</idno><idno type="wicri:Area/Main/Exploration">000020</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">A Diverse Virome of Leafroll-Infected Grapevine Unveiled by dsRNA Sequencing.</title><author><name sortKey="Fall, Mamadou L" sort="Fall, Mamadou L" uniqKey="Fall M" first="Mamadou L" last="Fall">Mamadou L. Fall</name><affiliation wicri:level="1"><nlm:affiliation>Saint-Jean-sur-Richelieu Research and Development Centre, Agriculture and Agri-Food Canada, St-Jean-sur-Richelieu, Quebec, QC J3B 3E6, Canada.</nlm:affiliation><country xml:lang="fr">Canada</country><wicri:regionArea>Saint-Jean-sur-Richelieu Research and Development Centre, Agriculture and Agri-Food Canada, St-Jean-sur-Richelieu, Quebec, QC J3B 3E6</wicri:regionArea><wicri:noRegion>QC J3B 3E6</wicri:noRegion></affiliation></author><author><name sortKey="Xu, Dong" sort="Xu, Dong" uniqKey="Xu D" first="Dong" last="Xu">Dong Xu</name><affiliation wicri:level="1"><nlm:affiliation>Saint-Jean-sur-Richelieu Research and Development Centre, Agriculture and Agri-Food Canada, St-Jean-sur-Richelieu, Quebec, QC J3B 3E6, Canada.</nlm:affiliation><country xml:lang="fr">Canada</country><wicri:regionArea>Saint-Jean-sur-Richelieu Research and Development Centre, Agriculture and Agri-Food Canada, St-Jean-sur-Richelieu, Quebec, QC J3B 3E6</wicri:regionArea><wicri:noRegion>QC J3B 3E6</wicri:noRegion></affiliation></author><author><name sortKey="Lemoyne, Pierre" sort="Lemoyne, Pierre" uniqKey="Lemoyne P" first="Pierre" last="Lemoyne">Pierre Lemoyne</name><affiliation wicri:level="1"><nlm:affiliation>Saint-Jean-sur-Richelieu Research and Development Centre, Agriculture and Agri-Food Canada, St-Jean-sur-Richelieu, Quebec, QC J3B 3E6, Canada.</nlm:affiliation><country xml:lang="fr">Canada</country><wicri:regionArea>Saint-Jean-sur-Richelieu Research and Development Centre, Agriculture and Agri-Food Canada, St-Jean-sur-Richelieu, Quebec, QC J3B 3E6</wicri:regionArea><wicri:noRegion>QC J3B 3E6</wicri:noRegion></affiliation></author><author><name sortKey="Moussa, Issam E Ben" sort="Moussa, Issam E Ben" uniqKey="Moussa I" first="Issam E Ben" last="Moussa">Issam E Ben Moussa</name><affiliation wicri:level="1"><nlm:affiliation>Saint-Jean-sur-Richelieu Research and Development Centre, Agriculture and Agri-Food Canada, St-Jean-sur-Richelieu, Quebec, QC J3B 3E6, Canada.</nlm:affiliation><country xml:lang="fr">Canada</country><wicri:regionArea>Saint-Jean-sur-Richelieu Research and Development Centre, Agriculture and Agri-Food Canada, St-Jean-sur-Richelieu, Quebec, QC J3B 3E6</wicri:regionArea><wicri:noRegion>QC J3B 3E6</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:affiliation>Département de Biologie, Université de Sherbrooke, Sherbrooke, QC J1K 2R1, Canada.</nlm:affiliation><country xml:lang="fr">Canada</country><wicri:regionArea>Département de Biologie, Université de Sherbrooke, Sherbrooke, QC J1K 2R1</wicri:regionArea><wicri:noRegion>QC J1K 2R1</wicri:noRegion></affiliation></author><author><name sortKey="Beaulieu, Carole" sort="Beaulieu, Carole" uniqKey="Beaulieu C" first="Carole" last="Beaulieu">Carole Beaulieu</name><affiliation wicri:level="1"><nlm:affiliation>Département de Biologie, Université de Sherbrooke, Sherbrooke, QC J1K 2R1, Canada.</nlm:affiliation><country xml:lang="fr">Canada</country><wicri:regionArea>Département de Biologie, Université de Sherbrooke, Sherbrooke, QC J1K 2R1</wicri:regionArea><wicri:noRegion>QC J1K 2R1</wicri:noRegion></affiliation></author><author><name sortKey="Carisse, Odile" sort="Carisse, Odile" uniqKey="Carisse O" first="Odile" last="Carisse">Odile Carisse</name><affiliation wicri:level="1"><nlm:affiliation>Saint-Jean-sur-Richelieu Research and Development Centre, Agriculture and Agri-Food Canada, St-Jean-sur-Richelieu, Quebec, QC J3B 3E6, Canada.</nlm:affiliation><country xml:lang="fr">Canada</country><wicri:regionArea>Saint-Jean-sur-Richelieu Research and Development Centre, Agriculture and Agri-Food Canada, St-Jean-sur-Richelieu, Quebec, QC J3B 3E6</wicri:regionArea><wicri:noRegion>QC J3B 3E6</wicri:noRegion></affiliation></author></analytic><series><title level="j">Viruses</title><idno type="eISSN">1999-4915</idno><imprint><date when="2020" type="published">2020</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Quebec is the third-largest wine grape producing province in Canada, and the industry is constantly expanding. Traditionally, 90% of the grapevine cultivars grown in Quebec were winter hardy and largely dominated by interspecific hybrid <i>Vitis</i> sp. cultivars. Over the years, the winter protection techniques adopted by growers and climate changes have offered an opportunity to establish <i>V. vinifera L</i>. cultivars (e.g., Pinot noir). We characterized the virome of leafroll-infected interspecific hybrid cultivar and compared it to the virome of <i>V. vinifera</i> cultivar to support and facilitate the transition of the industry. A dsRNA sequencing method was used to sequence symptomatic and asymptomatic grapevine leaves of different cultivars. The results suggested a complex virome in terms of composition, abundance, richness, and phylogenetic diversity. Three viruses, grapevine Rupestris stem pitting-associated virus, grapevine leafroll-associated virus (GLRaV) 3 and 2 and hop stunt viroid (HSVd) largely dominated the virome. However, their presence and abundance varied among grapevine cultivars. The symptomless grapevine cultivar Vidal was frequently infected by multiple virus and viroid species and different strains of the same virus, including GLRaV-3 and 2. Our data show that viruses and viroids associated with the highest number of grapevines expressing symptoms included HSVd, GLRaV-3 and GLRaV-2, in gradient order. However, co-occurrence analysis revealed that the presence of GLRaV species was randomly associated with the development of virus-like symptoms. These findings and their implications for grapevine leafroll disease management are discussed.</div></front></TEI><pubmed><MedlineCitation Status="In-Process" Owner="NLM"><PMID Version="1">33050079</PMID><DateRevised><Year>2020</Year><Month>11</Month><Day>03</Day></DateRevised><Article PubModel="Electronic"><Journal><ISSN IssnType="Electronic">1999-4915</ISSN><JournalIssue CitedMedium="Internet"><Volume>12</Volume><Issue>10</Issue><PubDate><Year>2020</Year><Month>10</Month><Day>08</Day></PubDate></JournalIssue><Title>Viruses</Title><ISOAbbreviation>Viruses</ISOAbbreviation></Journal><ArticleTitle>A Diverse Virome of Leafroll-Infected Grapevine Unveiled by dsRNA Sequencing.</ArticleTitle><ELocationID EIdType="pii" ValidYN="Y">E1142</ELocationID><ELocationID EIdType="doi" ValidYN="Y">10.3390/v12101142</ELocationID><Abstract><AbstractText>Quebec is the third-largest wine grape producing province in Canada, and the industry is constantly expanding. Traditionally, 90% of the grapevine cultivars grown in Quebec were winter hardy and largely dominated by interspecific hybrid <i>Vitis</i> sp. cultivars. Over the years, the winter protection techniques adopted by growers and climate changes have offered an opportunity to establish <i>V. vinifera L</i>. cultivars (e.g., Pinot noir). We characterized the virome of leafroll-infected interspecific hybrid cultivar and compared it to the virome of <i>V. vinifera</i> cultivar to support and facilitate the transition of the industry. A dsRNA sequencing method was used to sequence symptomatic and asymptomatic grapevine leaves of different cultivars. The results suggested a complex virome in terms of composition, abundance, richness, and phylogenetic diversity. Three viruses, grapevine Rupestris stem pitting-associated virus, grapevine leafroll-associated virus (GLRaV) 3 and 2 and hop stunt viroid (HSVd) largely dominated the virome. However, their presence and abundance varied among grapevine cultivars. The symptomless grapevine cultivar Vidal was frequently infected by multiple virus and viroid species and different strains of the same virus, including GLRaV-3 and 2. Our data show that viruses and viroids associated with the highest number of grapevines expressing symptoms included HSVd, GLRaV-3 and GLRaV-2, in gradient order. However, co-occurrence analysis revealed that the presence of GLRaV species was randomly associated with the development of virus-like symptoms. These findings and their implications for grapevine leafroll disease management are discussed.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Fall</LastName><ForeName>Mamadou L</ForeName><Initials>ML</Initials><Identifier Source="ORCID">0000-0002-1747-6481</Identifier><AffiliationInfo><Affiliation>Saint-Jean-sur-Richelieu Research and Development Centre, Agriculture and Agri-Food Canada, St-Jean-sur-Richelieu, Quebec, QC J3B 3E6, Canada.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Xu</LastName><ForeName>Dong</ForeName><Initials>D</Initials><AffiliationInfo><Affiliation>Saint-Jean-sur-Richelieu Research and Development Centre, Agriculture and Agri-Food Canada, St-Jean-sur-Richelieu, Quebec, QC J3B 3E6, Canada.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Lemoyne</LastName><ForeName>Pierre</ForeName><Initials>P</Initials><AffiliationInfo><Affiliation>Saint-Jean-sur-Richelieu Research and Development Centre, Agriculture and Agri-Food Canada, St-Jean-sur-Richelieu, Quebec, QC J3B 3E6, Canada.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Moussa</LastName><ForeName>Issam E Ben</ForeName><Initials>IEB</Initials><AffiliationInfo><Affiliation>Saint-Jean-sur-Richelieu Research and Development Centre, Agriculture and Agri-Food Canada, St-Jean-sur-Richelieu, Quebec, QC J3B 3E6, Canada.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Département de Biologie, Université de Sherbrooke, Sherbrooke, QC J1K 2R1, Canada.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Beaulieu</LastName><ForeName>Carole</ForeName><Initials>C</Initials><AffiliationInfo><Affiliation>Département de Biologie, Université de Sherbrooke, Sherbrooke, QC J1K 2R1, Canada.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Carisse</LastName><ForeName>Odile</ForeName><Initials>O</Initials><AffiliationInfo><Affiliation>Saint-Jean-sur-Richelieu Research and Development Centre, Agriculture and Agri-Food Canada, St-Jean-sur-Richelieu, Quebec, QC J3B 3E6, Canada.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2020</Year><Month>10</Month><Day>08</Day></ArticleDate></Article><MedlineJournalInfo><Country>Switzerland</Country><MedlineTA>Viruses</MedlineTA><NlmUniqueID>101509722</NlmUniqueID><ISSNLinking>1999-4915</ISSNLinking></MedlineJournalInfo><CitationSubset>IM</CitationSubset><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="Y">Vitis vinifera</Keyword><Keyword MajorTopicYN="Y">dsRNA extraction</Keyword><Keyword MajorTopicYN="Y">grapevine leafroll disease</Keyword><Keyword MajorTopicYN="Y">interspecific hybrid</Keyword><Keyword MajorTopicYN="Y">viromics</Keyword><Keyword MajorTopicYN="Y">virus co-occurrence</Keyword><Keyword MajorTopicYN="Y">virus epidemiology</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2020</Year><Month>08</Month><Day>28</Day></PubMedPubDate><PubMedPubDate PubStatus="revised"><Year>2020</Year><Month>09</Month><Day>30</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2020</Year><Month>10</Month><Day>06</Day></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2020</Year><Month>10</Month><Day>14</Day><Hour>1</Hour><Minute>3</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2020</Year><Month>10</Month><Day>15</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2020</Year><Month>10</Month><Day>15</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>epublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">33050079</ArticleId><ArticleId IdType="pii">v12101142</ArticleId><ArticleId IdType="doi">10.3390/v12101142</ArticleId><ArticleId IdType="pmc">PMC7599845</ArticleId></ArticleIdList><ReferenceList><Reference><Citation>Plant Dis. 2019 Mar;103(3):509-518</Citation><ArticleIdList><ArticleId IdType="pubmed">30667323</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant Dis. 2017 Dec;101(12):2088-2097</Citation><ArticleIdList><ArticleId IdType="pubmed">30677387</ArticleId></ArticleIdList></Reference><Reference><Citation>PLoS One. 2013 Jun 05;8(6):e64194</Citation><ArticleIdList><ArticleId IdType="pubmed">23755117</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant Dis. 1997 Jun;81(6):625-628</Citation><ArticleIdList><ArticleId IdType="pubmed">30861847</ArticleId></ArticleIdList></Reference><Reference><Citation>Virol J. 2015 Oct 20;12:171</Citation><ArticleIdList><ArticleId IdType="pubmed">26482551</ArticleId></ArticleIdList></Reference><Reference><Citation>Front Microbiol. 2013 Apr 16;4:82</Citation><ArticleIdList><ArticleId IdType="pubmed">23596440</ArticleId></ArticleIdList></Reference><Reference><Citation>PLoS One. 2016 Feb 26;11(2):e0149666</Citation><ArticleIdList><ArticleId IdType="pubmed">26919614</ArticleId></ArticleIdList></Reference><Reference><Citation>J Virol Methods. 2016 Oct;236:35-40</Citation><ArticleIdList><ArticleId IdType="pubmed">27387642</ArticleId></ArticleIdList></Reference><Reference><Citation>Front Microbiol. 2018 Aug 29;9:1782</Citation><ArticleIdList><ArticleId IdType="pubmed">30210456</ArticleId></ArticleIdList></Reference><Reference><Citation>Virology. 2014 Dec;471-473:54-60</Citation><ArticleIdList><ArticleId IdType="pubmed">25461531</ArticleId></ArticleIdList></Reference><Reference><Citation>Front Microbiol. 2013 Apr 24;4:94</Citation><ArticleIdList><ArticleId IdType="pubmed">23630520</ArticleId></ArticleIdList></Reference><Reference><Citation>PLoS One. 2016 Dec 13;11(12):e0167966</Citation><ArticleIdList><ArticleId IdType="pubmed">27959951</ArticleId></ArticleIdList></Reference><Reference><Citation>Bioinformatics. 2014 Aug 1;30(15):2114-20</Citation><ArticleIdList><ArticleId IdType="pubmed">24695404</ArticleId></ArticleIdList></Reference><Reference><Citation>Arch Virol. 2018 Nov;163(11):3105-3111</Citation><ArticleIdList><ArticleId IdType="pubmed">30043203</ArticleId></ArticleIdList></Reference><Reference><Citation>Virol J. 2018 Aug 13;15(1):127</Citation><ArticleIdList><ArticleId IdType="pubmed">30103767</ArticleId></ArticleIdList></Reference><Reference><Citation>Phytopathology. 2015 Jun;105(6):758-63</Citation><ArticleIdList><ArticleId IdType="pubmed">25689518</ArticleId></ArticleIdList></Reference><Reference><Citation>Phytopathology. 2009 Oct;99(10):1177-84</Citation><ArticleIdList><ArticleId IdType="pubmed">19740031</ArticleId></ArticleIdList></Reference><Reference><Citation>Phytopathology. 2012 Jul;102(7):717-23</Citation><ArticleIdList><ArticleId IdType="pubmed">22439861</ArticleId></ArticleIdList></Reference><Reference><Citation>PLoS One. 2011;6(6):e19992</Citation><ArticleIdList><ArticleId IdType="pubmed">21687704</ArticleId></ArticleIdList></Reference><Reference><Citation>J Virol Methods. 2016 Sep;235:112-118</Citation><ArticleIdList><ArticleId IdType="pubmed">27246908</ArticleId></ArticleIdList></Reference><Reference><Citation>Virus Res. 2012 Jan;163(1):262-8</Citation><ArticleIdList><ArticleId IdType="pubmed">22036729</ArticleId></ArticleIdList></Reference><Reference><Citation>Ecol Lett. 2020 Jul;23(7):1050-1063</Citation><ArticleIdList><ArticleId IdType="pubmed">32429003</ArticleId></ArticleIdList></Reference><Reference><Citation>Nucleic Acids Res. 2019 Jul 2;47(W1):W256-W259</Citation><ArticleIdList><ArticleId IdType="pubmed">30931475</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant Dis. 2019 Jun;103(6):1275-1285</Citation><ArticleIdList><ArticleId IdType="pubmed">30932733</ArticleId></ArticleIdList></Reference><Reference><Citation>Virus Genes. 2011 Aug;43(1):102-10</Citation><ArticleIdList><ArticleId IdType="pubmed">21487783</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant Dis. 2017 Aug;101(8):1489-1499</Citation><ArticleIdList><ArticleId IdType="pubmed">30678581</ArticleId></ArticleIdList></Reference><Reference><Citation>Arch Virol. 2018 Nov;163(11):2937-2946</Citation><ArticleIdList><ArticleId IdType="pubmed">30033497</ArticleId></ArticleIdList></Reference></ReferenceList></PubmedData></pubmed><affiliations><list><country><li>Canada</li></country></list><tree><country name="Canada"><noRegion><name sortKey="Fall, Mamadou L" sort="Fall, Mamadou L" uniqKey="Fall M" first="Mamadou L" last="Fall">Mamadou L. Fall</name></noRegion><name sortKey="Beaulieu, Carole" sort="Beaulieu, Carole" uniqKey="Beaulieu C" first="Carole" last="Beaulieu">Carole Beaulieu</name><name sortKey="Carisse, Odile" sort="Carisse, Odile" uniqKey="Carisse O" first="Odile" last="Carisse">Odile Carisse</name><name sortKey="Lemoyne, Pierre" sort="Lemoyne, Pierre" uniqKey="Lemoyne P" first="Pierre" last="Lemoyne">Pierre Lemoyne</name><name sortKey="Moussa, Issam E Ben" sort="Moussa, Issam E Ben" uniqKey="Moussa I" first="Issam E Ben" last="Moussa">Issam E Ben Moussa</name><name sortKey="Moussa, Issam E Ben" sort="Moussa, Issam E Ben" uniqKey="Moussa I" first="Issam E Ben" last="Moussa">Issam E Ben Moussa</name><name sortKey="Xu, Dong" sort="Xu, Dong" uniqKey="Xu D" first="Dong" last="Xu">Dong Xu</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Bois/explor/GrapevineDiseaseV1/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000110 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 000110 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Bois
|area= GrapevineDiseaseV1
|flux= Main
|étape= Exploration
|type= RBID
|clé= pubmed:33050079
|texte= A Diverse Virome of Leafroll-Infected Grapevine Unveiled by dsRNA Sequencing.
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Exploration/RBID.i -Sk "pubmed:33050079" \
| HfdSelect -Kh $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd \
| NlmPubMed2Wicri -a GrapevineDiseaseV1
| This area was generated with Dilib version V0.6.37. |  |